Lobelia

True-scale silhouettes from herbarium specimens, and leaf measurement.

Undergraduate research at Kent State University in Dr. Andrea Case’s lab, on Lobelia.

Silhouettes (2018–19)

Paper · Download the set (CC BY 4.0)

I restored digitized Lobelia herbarium specimens in Photoshop and turned each plant into a silhouette: 39 silhouettes across 27 species. They form the morphology panel of the phylogeny figure (Fig. 2B) in Godden et al. 2025 (Godden et al., 2025).

Twenty-seven black plant silhouettes in a row on a common baseline, ordered left to right from shortest to tallest, from Lobelia feayana at 14 cm to Lobelia brevifolia at 134 cm, with a 50 cm scale bar.
All 27 species at true scale, from L. feayana (14 cm) to L. brevifolia (134 cm).

For each sheet I unfolded pressed leaves, rejoined broken stems, and removed labels, tape and colour cards, then converted the restored plant to a mask. Nothing was added from other specimens, so a part missing from the sheet is missing from its silhouette. Each file has a 10 cm bar taken from the sheet’s own ruler, which is how the plate above puts every plant on one scale.

Heights are those of the mounted specimen, not the species. A sheet only holds what fits on it: the three L. cardinalis are 24–52 cm, against a published 122–152 cm for the species.

All 39 specimens on one scale. Hover for species and size; scroll sideways for the tallest.

Arnold, J. (2026). Lobelia silhouettes: digitally restored whole-plant outlines from herbarium specimens (2018–2019). Zenodo. 10.5281/zenodo.21764522

The 39 silhouettes, the plate and a table of heights, under CC BY 4.0. The specimen photographs belong to the herbaria that hold them and are not included.

Leaf measurement

Part of NSF award DEB-2015606, which uses Lobelia sect. Lobelia to study why close relatives do or do not grow together.

Leaf shape differs between close relatives and is tied to how a leaf works: light interception, temperature, water supply (Nicotra et al., 2011; Tsukaya, 2018). Many species in the section grow as basal rosettes, which press flat into a pile of overlapping leaves, so the vouchers were taken apart and photographed with the leaves laid out separately. I cropped each leaf in ImageJ, leaving out those too folded or torn to measure, and measured area and perimeter with the LeafArea R package against a 1 cm scale.

In exploratory plots, the perimeter–area relationship looked different between species and between collection sites of L. elongata. This was not tested statistically. The photographs and measurements belong to the Case lab and are not published here.

References

2025

  1. Population-level phylogenomic analysis yields insights into species cohesion and population substructure of Lobelia section Lobelia (Campanulaceae)
    Grant T. Godden, Andrew A. Crowl, Stephen R. Patten, and 11 more authors
    Molecular Phylogenetics and Evolution, 2025
    Funded in part by NSF DEB-2015606, the award the leaf-measurement work was part of. The silhouettes are Fig. 2B.

2018

  1. A Consideration of Leaf Shape Evolution in the Context of the Primary Function of the Leaf as a Photosynthetic Organ
    Hirokazu Tsukaya
    In Advances in Photosynthesis and Respiration, 2018

2011

  1. The evolution and functional significance of leaf shape in the angiosperms
    Adrienne B. Nicotra, Andrea Leigh, C. Kevin Boyce, and 4 more authors
    Functional Plant Biology, 2011